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. Author manuscript; available in PMC: 2020 Jun 27.
Published in final edited form as: Cell. 2019 May 30;178(1):176–189.e15. doi: 10.1016/j.cell.2019.05.003

Figure 5. LDHA-associated lactate inhibits RLR signaling in vivo.

Figure 5.

A, Q-PCR analysis of IFN-β and IFN-α expression in lung from mice fasted overnight and then treated with high glucose (1.5 g/kg) or low glucose (0.2 g/kg) with or without following injection of sodium lactate (1 g/kg) and infected with VSV (2×107 pfu/g). B, ELISA analysis of IFN- β in sera of Ldha+/+ and Ldha−/− mice intraperitoneal injected with VSV (2×107 pfu/g). C and D, Q-PCR analysis of IFN- β expression in spleen (C) and VSV replication in different organs (D) from Ldha+/+ and Ldha−/− mice infected with VSV. E, Hematoxylin and eosin (HE) staining of lung sections in Ldha+/+ and Ldha−/− mice described in D. Scale bar, 100 mm. F and G, Analysis of lactate secretion (F) and IFN-β production (G) in supernatants of peritoneal macrophages generated from Ldha+/+ and Ldha−/− mice and treated with Poly(I:C) transfection, Sev or VSV infection. H, ELISA analysis of IFN-β production in supernatants of peritoneal macrophages generated from Ldha−/− mice and then added with or without lactate (10 mM) before VSV infection. I, Q-PCR analysis of type-I IFN expression in lung from mice injected with or without sodium oxamate (750 mg/kg) and then challenged by VSV (2×107 pfu/g). J, Q-PCR analysis of IFN-β mRNA expression in lung tissue from mice injected with or without sodium oxamate and infected with HSV. Data are means±SD. **p < 0.01. See also Figure S6.