Table 1:
Biofluid Sample Preparationa
| Biofluid | Required sample handling |
|---|---|
| Urine | Add deuterated phosphate buffer to 0.2–0.4 mL urine |
| Blood/plasma/serum | Add deuterium oxide (to lock) Add acetonitrile (for protein precipitation) Add methanol/chloroform extraction (for lipid extraction) |
| Cerebrospinal fluid | Add deuterium oxide to 0.5 mL of CSF |
| Expressed prostatic secretions | Add deuterium oxide to 0.03 – 0.10 mL of EPS |
| Bile | Add deuterium methanol to 0.5 mL of bile |
| Bronchoalveolar lavage fluid | Add deuterium oxide to 0.5 mL of BALF |
| Tissue | Add 0.01 mL of deuterium oxide to 3–10 g of tissue in MAS rotor Add perchloric acid extraction on 20–200 g frozen tissue Add methanol/chloroform extraction to 20–200 g frozen tissue |
a
Adapted from Spratlin et al. (2009)