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. 2019 Jun 3;20(2):1443–1450. doi: 10.3892/mmr.2019.10319

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Copyright: © Chen et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — PEDF induces sestrin2 gene expression and activates sestrin2 transcription through p53. (A) HUVECs were treated with PEDF for 0, 4, 8, 12 and 16 h, and sestrin2 mRNA expression was detected by RT-qPCR. *P<0.05 vs. 0 h. (B) Western blot analysis of sestrin2 protein expression in PEDF-treated HUVECs at different time points. (C) Semi-quantitative analysis of blot in (B). *P<0.05 vs. 0 h. (D) p53 siRNA-transfected HUVECs were treated with PEDF for 12 h and sestrin2 was detected by RT-qPCR. *P<0.05 vs. PEDF-treated group. HUVECs, human umbilical vein endothelial cells; PEDF, pigment epithelium-derived factor; si, small interfering; RT-qPCR, reverse transcription-quantitative polymerase chain reaction.