Figure 6.
(A) Serum IgE levels were measured by ELISA. (B) Western blot revealed protein level changes in the expression of Nrf2, p-Nrf2, HO-1, p-JAK1, JAK1, p-STAT3 and STAT3. Tubulin was used as an internal reference. (C) Reverse transcription-quantitative PCR assay revealed mRNA level changes in the expression of (C) IL-6, (D) IL-1β and (E) TNF-α. Results are expressed as the mean ± SEM (n=8). *P<0.05, **P<0.01, ***P<0.001 and ****P<0.0001. One-way ANOVA analysis followed by Dunnett's post hoc test. DNCB, 2,4-dinitrochlorobenzene; SFN, sulforaphane; Vehicle, vehicle + PBS-treated group; AD, DNCB + PBS-treated group; AD + SFN2.5, DNCB + SFN (2.5 mg/kg i.p.)-treated group; AD+SFN5, DNCB + SFN (5 mg/kg i.p.)-treated group; AD + SFN10, DNCB + SFN (10 mg/kg i.p.)-treated group.
