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. 2019 Jun 25;20(2):1725–1735. doi: 10.3892/mmr.2019.10421

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Copyright: © Xu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 5. — Neuron-specific markers expressed in rLV-miR and rLV-miR-153 cells. (A) Relative PRX5 and NSE expression as detected by regular PCR (a). Significance was determined by comparing the miR-153 overexpression group to the control with unpaired t-test; n=3 (b). (B) The protein expression of PRX5, NSE, SNAP23, SNAP25 and NeuN was analyzed by western blotting (a). Band density was determined in the miR-153 overexpression group and control, the error bars represent standard deviation (b). (C) The protein expression of (a) NSE was analyzed by immunofluorescence staining. Scale bar 400 µm. (C) The protein expression of (b) SNAP23 and (c) SNAP25 was analyzed by immunofluorescence staining. NSE (a), SNAP23 (b) and SNAP25 (c) were found to be significantly increased in rLV-miR-153-overexpressing cells. Arrows indicate positively stained cells. Significance was determined by comparing NSE, SNAP23 and SNAP25 protein expression in the rLV-miR-153-overexpressing group to control with multiple unpaired t-tests; n=5. Error bars represent standard deviation. **P<0.01. Cells exhibiting a strong orange color were identified as positive cells expressing NSE, SNAP23 and SNAP25 (arrows). (C-d) Five visual fields of each cell group were examined and the positive percentage of cells was calculated. NSE, neuron-specific enolase; PRX5, peroxiredoxin 5; SNAP, N-ethylmaleimide-sensitive fusion attachment protein; NeuN, neuronal nuclei.

Figure 5. — Neuron-specific markers expressed in rLV-miR and rLV-miR-153 cells. (A) Relative PRX5 and NSE expression as detected by regular PCR (a). Significance was determined by comparing the miR-153 overexpression group to the control with unpaired t-test; n=3 (b). (B) The protein expression of PRX5, NSE, SNAP23, SNAP25 and NeuN was analyzed by western blotting (a). Band density was determined in the miR-153 overexpression group and control, the error bars represent standard deviation (b). (C) The protein expression of (a) NSE was analyzed by immunofluorescence staining. Scale bar 400 µm. (C) The protein expression of (b) SNAP23 and (c) SNAP25 was analyzed by immunofluorescence staining. NSE (a), SNAP23 (b) and SNAP25 (c) were found to be significantly increased in rLV-miR-153-overexpressing cells. Arrows indicate positively stained cells. Significance was determined by comparing NSE, SNAP23 and SNAP25 protein expression in the rLV-miR-153-overexpressing group to control with multiple unpaired t-tests; n=5. Error bars represent standard deviation. **P<0.01. Cells exhibiting a strong orange color were identified as positive cells expressing NSE, SNAP23 and SNAP25 (arrows). (C-d) Five visual fields of each cell group were examined and the positive percentage of cells was calculated. NSE, neuron-specific enolase; PRX5, peroxiredoxin 5; SNAP, N-ethylmaleimide-sensitive fusion attachment protein; NeuN, neuronal nuclei.