Table I.
Primers used in the present study.
| Gene | Sequence | Product size |
|---|---|---|
| NLRP3 | ||
| Upstream | F-5′-AAGCACCTGTTGTGCAATCTGAAG-3′ | 103 bp |
| Downstream | R-5′-GGGAATGGCTGGTGCTCAATAC-3′ | |
| ASC | ||
| Upstream | F-5′-TGACGGATGAGCAGTACCAG-3′ | 151 bp |
| Downstream | R-5′-TCCTCCACCAGGTAGGACTG-3′ | |
| Caspase-1 | ||
| Upstream | F-5′-GCCTGTTCCTGTGATGTGGA-3′ | 175 bp |
| Downstream | R-5′-TTCACTTCCTGCCCACAGAC-3′ | |
| β-actin | ||
| Upstream | F-5′-CCTTCCTGGGCATGGAGTCCTG-3′ | 202 bp |
| Downstream | R-5′-GGAGCAATGATCTTGATCTTC-3′ | |
NLRP3, nucleotide-binding oligomerization domain, leucine rich repeat, and pyrin domain-containing protein 3; ASC, apoptosis-associated speck-like protein containing a CARD; F, forward; R, reverse.