Figure 5.
Influence of nucleus pulposus cells on the migration of VECs in the co-culture system. (A) In the co-culture system, before adding AMD3100, the number of transmembrane VECs increased as stromal cell derived factor increased in the Down, D and Up groups. After adding AMD3100, the number of transmembrane VECs decreased in all three groups. Magnification, ×200. (B) Statistical results, showing that there was a significant difference in the number of transmembrane VECs between each group before adding AMD3100. After adding AMD3100, the number of transmembrane VECs in the D and Up groups decreased significantly. There was no significant difference in the Down group before or after adding AMD3100. *P<0.05, **P<0.01, ***P<0.001. VECs, vascular endothelial cells; Down, downregulation; D, degeneration; Up, upregulation; AMD3100-, without adding AMD3100 into the co-culture system; AMD3100+, adding AMD3100 into the co-culture system.