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. 2019 Jun 4;20(2):1093–1102. doi: 10.3892/mmr.2019.10330

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Copyright: © Yuan et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 6. — Effects of TGF-β₁ in culture medium on the changes of extracellular matrix components. Cardiac fibroblasts were treated with HG (40 mM) in the presence or absence of TGF-β₁ (5, 10, 20 ng/ml) for 48 h at 37°C. (A) After different treatments, the protein expression of Col-I, Col-III, MMP-2 and MMP-9 was evaluated by western blot analysis. (B) The changes in the mRNA levels of Col-I, Col-III, MMP-2 and MMP-9 were measured by RT-qPCR, and the mRNA levels were normalized to β-actin, which was used as the housekeeping gene. (C) The cell supernatants were collected for the determination of Col-I, Col-III, MMP2 and MMP9 expression using ELISA. *P<0.05, **P<0.01 vs. the Control; ^#P<0.05, ^##P<0.01, ^###P<0.001 vs. HG (n≥8). Col, collagen; SMA, smooth muscle actin; MMP, matrix metallopeptidase; TGF, transforming growth factor; HG, high glucose.

Figure 6. — Effects of TGF-β₁ in culture medium on the changes of extracellular matrix components. Cardiac fibroblasts were treated with HG (40 mM) in the presence or absence of TGF-β₁ (5, 10, 20 ng/ml) for 48 h at 37°C. (A) After different treatments, the protein expression of Col-I, Col-III, MMP-2 and MMP-9 was evaluated by western blot analysis. (B) The changes in the mRNA levels of Col-I, Col-III, MMP-2 and MMP-9 were measured by RT-qPCR, and the mRNA levels were normalized to β-actin, which was used as the housekeeping gene. (C) The cell supernatants were collected for the determination of Col-I, Col-III, MMP2 and MMP9 expression using ELISA. *P<0.05, **P<0.01 vs. the Control; ^#P<0.05, ^##P<0.01, ^###P<0.001 vs. HG (n≥8). Col, collagen; SMA, smooth muscle actin; MMP, matrix metallopeptidase; TGF, transforming growth factor; HG, high glucose.