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. 2019 May 31;20(2):1221–1229. doi: 10.3892/mmr.2019.10317

Figure 2.

Figure 2.

SIRT1 is a direct target of miR-217. (A) Interaction between miR-217 and the 3′-UTR of SIRT1 predicted using TargetScan. (B) Level of miR-217 in neurons transfected with miR-217 mimic or mimic control as determined by RT-qPCR. (C) Luciferase activity of reporters containing the WT or MUT SIRT1 3′-UTR following transfection with miR-217 mimic or mimic control. Data are presented as the mean ± standard deviation of three independent experiments. **P<0.01 vs. mimic control. (D) Protein and (E) mRNA expression of SIRT1 in neurons following OGD/R treatment as determined by western blot and RT-qPCR analyses, respectively. Data are presented as the mean ± standard deviation. **P<0.01 vs. Control. RT-qPCR, reverse transcription-quantitative PCR; SIRT1, sirtuin 1; 3′-UTR, 3′-untranslated region; miR-217, microRNA-217; WT, wild-type; MUT, mutated; OGD/R, oxygen-glucose deprivation and reoxygenation.