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. 2019 May 31;20(2):1221–1229. doi: 10.3892/mmr.2019.10317

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Copyright: © Rao et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — Effects of miR-217 inhibitor on OGD/R-induced neuronal injury. Neurons were transfected with miR-217 inhibitor, inhibitor control or miR-217 inhibitor + sirtuin 1-siRNA for 24 h, followed by OGD/R treatment. (A) Cell viability was detected using a Cell Counting Kit-8 assay. (B) LDH release was measured by an LDH assay. (C and D) Cell apoptosis was determined using flow cytometry. Data are presented as the mean ± standard deviation. *P<0.05, **P<0.01 vs. control; ^#P<0.05, ^##P<0.01 vs. OGD/R; ^&P<0.05, ^&&P<0.01 vs. inhibitor. miR-217, microRNA-217; OGD/R, oxygen-glucose deprivation and reoxygenation; LDH, lactate dehydrogenase; siRNA, small interfering RNA; PI, propidium iodide.