Figure 7.

Effects of miR-217 inhibitor on SIRT1/AMPK-α/NF-κB pathway in OGD/R-treated neurons. Neurons were transfected with miR-217 inhibitor, inhibitor control or miR-217 inhibitor + SIRT1-siRNA for 24 h, followed by OGD/R treatment. Then, the (A) protein and (B) mRNA expression levels of SIRT1 were determined by western blot and reverse transcription-quantitative PCR analyses, respectively. Additionally, the protein levels of p-AMPK-α, AMPK-α, p65, and p-p65 were measured by western blotting. The ratios of (C) p-AMPK-α/AMPK-α and (D) p-p65/p65 normalized to β-actin were calculated and presented. Data are presented as the mean ± standard deviation. **P<0.01 vs. control; ^##P<0.01 vs. OGD/R; ^&P<0.05, ^&&P<0.01 vs. inhibitor. miR-217, microRNA-217; OGD/R, oxygen-glucose deprivation and reoxygenation; siRNA, small interfering RNA; SIRT1, sirtuin 1; p, phosphorylated; AMPK-α, AMP-activated protein kinase-α.