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. Author manuscript; available in PMC: 2019 Jul 12.
Published in final edited form as: J Neural Transm (Vienna). 2011 Jan 8;118(3):493–507. doi: 10.1007/s00702-010-0513-5

Fig. 3.

Fig. 3

Dicistronic construct designed to screen small molecule inhibitors of SNCA translation: Left panel the unique RNA stem-loop target in the 5′UTR of the transcript for SNCA (RNA predicted by MULTIFOLD (Cahill and Rogers 2008; Friedlich et al. 2007; Olivares et al. 2009; Zuker 1989). Right panel transfection-based screen with our dicistronic pIRES(SNCA) construct to identify SNCA 5′UTR directed inhibitors of luciferase reporter translation, but which selectively maintain GFP translation from the downstream IRES RNA structure. This RNA targeting technology has already identified translation inhibitors of APP mRNA (as a precedent for AD therapy (Rogers et al. 2002b; Bandyopadhyay et al. 2006b). Chemical structures of HTS inhibitors of the APP 5′UTR can be downloaded from PUBCHEM at the NCBI website as AID: 1285; we are now also screening for SNCA 5′UTR directed translation blockers (Broad Inst. Cambridge, MA, USA)