(A) Comparison of carbachol (CCh)-induced airway responsiveness in donors with and without asthma before and after infection with RV39. RV39 infection causes airway hyper-responsiveness to CCh only in PCLS from donors with a history of asthma. (B-E) IL25, TSLP, IL13, and IL-33 gene expression from RV39 infected PCLS. There are significant increases in the expression of IL-25, TSLP, and IL-13 in the PCLS from asthma donors compared to controls without asthma. (F, G) IL-33 and TSLP protein levels in supernatants from RV39 infected PCLS in donors with and without asthma. There are significantly increased TSLP protein levels in those donors with a history of asthma after infection with RV39.
* p<0.05
Adapted from Kennedy JL, Koziol-White CJ, Jeffus S, et al. Effects of rhinovirus 39 infection on airway hyperresponsiveness to carbachol in human airways precision cut lung slices. J Allergy Clin Immunol 2018; 141(5): 1888; with permission.