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. 2019 Mar 15;74(8):1180–1188. doi: 10.1093/gerona/glz067

Figure 2.

Figure 2.

Knockdown of YP170 synthesis reciprocally increases YP115/YP88 levels and vice versa. (A) Coomassie gel showing the effects of vit-5 and/or vit-6 RNAi on yolk protein (YP) levels. (B) Quantified data, fold change in YP levels. Note that vit-5 RNAi increases levels of YP115 and YP88, whereas vit-6 RNAi increases YP170 accumulation. Statistical comparisons are to L4440 control on the same day. (C) Effect of vit-5 and/or vit-6 RNAi on vit-2, vit-5 or vit-6 mRNA levels, compared to L4440 control on the same day. Note that vit-5 RNAi does not increase vit-6 mRNA levels and vit-6 RNAi does not increase vit-5 mRNA levels. (D) No change in the YP content per worm after vit-5 or vit-6 RNAi. (E) No effect of vit RNAi on total protein content. (F) Appearance of new protein bands after vit-5,-6 RNAi. All data are mean ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001.