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. 2019 Jul 1;13(7):e0007533. doi: 10.1371/journal.pntd.0007533

Fig 4. Biological activity of L. major-treated CXCL1 is diminished.

Fig 4

(A) Schematic representation of experimental design. (B) Recombinant murine CXCL1 was incubated with Lm lysate for 24h and CXCL1 levels was determined by ELISA. (C-E) BMDMs were treated Lm lysate, rm-CXCL1, or Lm lysate + rm-CXCL1 from (B) for indicated time-points and mRNA expression of pro-inflammatory genes Cxcl1 (C), Tnf (D) and Il6 (E) were evaluated by qPCR. Data are representative of at least three independent experiments.