KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit monoclonal anti-N-Cadherin | Cell Signaling | Cat#13116S; RRID: AB_2687616 |
| Rabbit monoclonal anti-SOX2 | Cell Signaling | Cat# 3579S; RRID: AB_2195767 |
| Mouse polyclonal anti-TUJ1 | BioLegend | Cat# 801201; RRID: AB_2313773 |
| Mouse monoclonal anti-PAX6 | Santa Cruz | Cat# sc-81649; RRID: AB_1127044 |
| Rabbit polyclonal anti-TBR2 | Abcam | Cat#ab23345; RRID: AB_778267 |
| Rabbit polyclonal MAP2 | Cell Signaling | Cat# 4542S; RRID: AB_10693782 |
| Rabbit monoclonal Musashi | Cell Signaling | Cat# 85652S; N/A |
| Rabbit polyclonal Nestin | BioLegend | Cat#841801; RRID: AB_2565467 |
| Rabbit monoclonal anti-GFAP | Cell Signaling | Cat# 12389S; RRID: AB_2631098 |
| Rabbit polyclonal anti-GFAP | Dako | Cat# ZO334; N/A |
| Rabbit polyclonal anti-Connexin-43 | Sigma Aldrich | Cat# C6219; RRID: AB_476857 |
| Chicken polyclonal anti-GFP | Abcam | Cat#ab13970; RRID: AB_300798 |
| Rabbit monoclonal anti-Ki67 | Cell Signaling | Cat#9129;N/A |
| Rabbit polyclonal Cleaved Caspase-3 | Cell Signaling | Cat#9661; RRID: AB_2341188 |
| Goat anti-mouse Alexa Fluor 488 | Thermo Fisher Scientific | Cat# A11001; RRID: AB_2534069 |
| Goat anti-rabbit Alexa Fluor 594 | Thermo Fisher Scientific | Cat# A11012; RRID: AB_2534079 |
| Goat anti-rabbit Alexa Fluor 568 | Thermo Fisher Scientific | Cat#A11036; RRID: AB_10563566 |
| Goat anti-chicken Alexa Fluor 488 | Thermo Fisher Scientific | Cat#A11039; RRID: AB_2534096 |
| Goat polyclonal anti-mouse IgG-HRP | Thermo Fisher Scientific | Cat# 31430; RRID: AB_228307 |
| Goat polyclonal anti-rabbit IgG-HRP | Thermo Fisher Scientific | Cat# 31460; RRID: AB_228341 |
| Goat polyclonal anti-rabbit IgG-Biotin | Jackson ImmunoResearch Laboratories | Cat# 111-065-003; RRID: AB_2337959 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| ROCK inhibitor (Y-27632 2HCI) | Selleck Chemicals | Cat# S1049; N/A |
| Rhod-2AM | Invitrogen | Cat# R-1244; N/A |
| Staurosporine | Sigma-Aldrich | Cat#S6942; N/A |
| Critical Commercial Assays | ||
| VECTASTAIN Elite ABC-HRP Kit | Vector Laboratories | Cat# PK-7200; RRID:AB_2336828 |
| Proteome Profiler Array | R&D Systems | Cat# ARY001B; N/A |
| Click-iT EdU Alexa Fluor 594 Imaging Kit | Thermo Fisher Scientific | Cat#C10339; N/A |
| QuantiFast SYBR Green PCR Kit | QIAGEN | Cat#204054; N/A |
| Cypridina Luciferase Glow Assay Kit | Pierce; Thermo Fisher Scientific | Cat#16170; N/A |
| Experimental Models: Cell Lines | ||
| Patient-Derived Glioma Stem Cells: 827, 1228, 211, 923, 308, 0728, 0320, 0517, 0607, 0810, and 1206 | National Institutes of Health and Weill Cornell Medicine/NYU Presbyterian Hospital | N/A |
| NIH-registered human H1 (WA01) embryonic stem cells | WiCell Research Institute | Cat# WA01; RRID: CVCL_9771 |
| NIH-registered human H9 (WA09) embryonic stem cells | WiCell Research Institute | Cat# WA09; RRID: CVCL_9773 |
| Recombinant DNA | ||
| pLenti PGK GFP Blast (w510-5) | Campeau et al., 2009 | Addgene Plasmid# 19069 |
| pGL4.50[luc2/CMV/Hygro] | Promega | Cat# E1310; N/A |
| pLenti-C-tRFP | OriGene | Cat# PS100074 |
| pLenti-C-TurboRFP-P2A-CLuc | Constructed in Lab | N/A |
| Software and Algorithms | ||
| ImageJ | NIH | https://imagej.nih.gov/ij/; RRID: nif-0000-30467 |
| Living Image | Living Image | Cat# 128113; http://www.perkinelmer.com/product/li-software-for-spectrum-1-seat-add-on-128113 |
| GraphPad Prism 7 | GraphPad | RRID: SCR_000306 |