Key Resources Table
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Monoclonal ANTI-FLAG M2 antibody produced in mouse | Sigma-Aldrich | F1804-1MG; RRID: AB_262044 |
| HA tag antibody (mouse) | GeneTex | GTX18181; RRID: AB_423116 |
| Anti-Mouse IgG (H&L) IRDye800 Conjugated Antibody | Rockland | RL-610-132-121; RRID: AB_220125 |
| Rabbit anti-HA Tag antibody affinity purified | ICL Lab | RHGT-45A-Z |
| Monoclonal ANTI-FLAG BioM2 antibody produced in mouse | Sigma-Aldrich | F9291-.2MG; RRID: AB_439698 |
| F(ab′)2-Goat anti-Rabbit IgG (H+L) Secondary Antibody, Qdot 705 | Life Technologies | Q11461MP; RRID: AB_2556474 |
| F(ab′)2-Goat anti-Rabbit IgG (H+L) Secondary Antibody, Qdot 605 | Life Technologies | Q11402MP; RRID: AB_10375594 |
| Anti-RPA32 | GeneTex | GTX22175, RRID:AB_384739 |
| Anti-RPA32 pS4/S8 | Bethyl Laboratories | A300-245A; RRID:AB_210547 |
| Anti-RPA32 pS33 | Bethyl Laboratories | A300-246A; RRID:AB_2180847 |
| Anti-Tubulin | Abcam | ab6046; RRID:AB_2210370 |
| Bacterial and Virus Strains | ||
| BL21(DE3) cells | Agilent | 230245 |
| ArcticExpress (DE3)RIL cells | Stratagene | 230193 |
| DH10bac cells | Thermo Fisher | 10361012 |
| Sf21 cells | Thermo Fisher | 11497013 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Streptavidin Atto647N | Atto-Tec | AD 647N-65 |
| Streptavidin Quantum Dot 705 | Life Technologies | Q10163MP |
| Streptavidin Quantum Dot 605 | Life Technologies | Q10103MP |
| Dynabeads M-280 Streptavidin | Life Technologies | 11205D |
| Bovine Serum Albumin (BSA) | Fisher Scientific | 9048-46-8 |
| DNase I | NEB | M0303S |
| Critical Commercial Assays | ||
| Q5 PCR Mutagenesis | NEB | E0554S |
| Bac-to-Bac Baculovirus Expression System | Life Technologies | N/A |
| Oligonucleotides | ||
| See Table S1 for a list of oligonucleotides used in this study | IDT | N/A |
| Recombinant DNA | ||
| pFastbac1 | Life Technologies | 10360014 |
| Lambda DNA | NEB | N3011S |
| pUC19 vector | NEB | N3041S |
| EXO1-avidity (pIF7), EXO1(D78A/D173A)-avidity (pIF418) | This study | Available upon request |
| RPA-His (pIF47), RPA-GFP-His (pIF48), RPAΔN-His (pIF537), pmRPA-GFP-His (pIF429), pmRPA-His (pIF430), RPA8A-His (pIF539), yRPA-His (pIF65) | This study | Available upon request |
| FLAG-BLM (pIF458), FLAG-BLM-K695A (pIF459), 3xHA-BLM (pIF527) | This study | Available upon request |
| FLAG-DNA2 (pIF494), FLAG-DNA2 (D277A) (pIF495) | This study | Available upon request |
| Human histone 3XHA-H2A (pIF131), H2B (pIF10), H3 (pIF11), H4 (pIF12) | This study | Available upon request |
| pEGFP-RPA32-WT (pIF528), pEGFP-RPA32-8D (pIF529), pEGFP-RPA32-8A (pIF530) | This study | Available upon request |
| pEf6-RPA32-WT (pIF532), pEf6-RPA32-8D (pIF533), pEf6-RPA32-8A (pIF534) | This study | Available upon request |
| pCAG-I-SceI (pIF540), pCAG-empty (pIF541) | This study | Available upon request |
| Software and Algorithms | ||
| FIJI (Fiji is just imageJ; v.1.47b) | ImageJ software | N/A |
| MATLAB (v.R2015b) | MathWorks | N/A |
| Custom FIJI and MATLAB scripts | This study | Available upon request |
| Other | ||
| Eclipse Ti-E Inverted TIRF Microscope | Nikon | MEA53100 |
| 60Å~ water-immersion objectivec(1.2NA) | Nikon | MRD07602 |
| 500 nm long-pass filter | Chroma | ET5001p |
| 638 nm dichroic beam splitter | Chroma | Custom Order |
| iXon X3 DU897 EMCCD cameras | Andor | 77026031 |
| Protein Lo-Bind Tubes | Eppendorf | 022431081 |
| Typhoon FLA 9500 | GE Healthcare | 28996943 |
| Optima XE ultracentrifuge | Beckman-Coulter | A99836 |
| Sonic Dismembrator Model 705 | Fisher Scientific | FB705110 |
| Ni-NTA Superflow Resin | QIAGEN | 30410 |
| HiTrap Q HP column | GE Healthcare | 17-1153-01 |
| Anti-FLAG M2 Affinity Gel | Sigma-Aldrich | A2220 |
| HiTrap SP HP column | GE Healthcare | 17115101 |
| HiTrap Heparin HP column | GE Healthcare | 17040601 |
| Superose 6 (10/300) | GE Healthcare | 17517201 |
| Superdex 200 (10/300) | GE Healthcare | 17517501 |
| Softlink Soft Release Avidin Resin | Promega | V201A |
| Sf21 insect cells (Grace’s media) | Invitrogen | B82101 |
| NovaCyte Flow Cytometer | Acea Biosciences | N/A |
| ViiA7 real-time PCR system | Thermo Fisher | N/A |
| Odyssey CLx imaging system | Li-Cor | N/A |