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. Author manuscript; available in PMC: 2019 Oct 9.
Published in final edited form as: Oncogene. 2019 Apr 9;38(28):5599–5611. doi: 10.1038/s41388-019-0811-9

Figure 4. Mst1r Signaling Upregulates Mst1 Expression.

Figure 4.

(A) KRC and CK epithelial cell lines derived from KC and KRC pancreata were screened for their relative expression of Mst1r and Mst1 by qRT-PCR. High levels of Mst1r expression correlated with similarly high levels of Mst1 expression in 3 out of 4 KRC epithelial cell lines. (B&C) Luciferase reporter assay results from murine pancreatic cell lines PanIN 4313 and 4964 LM. Cells were stimulated with 250ng/ml of Mst1 for 24–48h. A regulatory response element was located between −335bp and −485bp within the Mst1r promoter (p<0.0001, p =0.002). (D) Bone marrow-derived macrophages were exposed to 100 ng/mL Mst1 or vehicle. Q-RT-PCR demonstrated a significant increase in Mst1r transcript after Mst1 (p< 0.05). (E) Immunostaining in 9m pancreata demonstrate increased Mst1 expression in high grade PanIN lesions in KRC versus KC mice.