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Reduction of PT638 by NfsA and NfsBa
| Enzyme | kcat (s−1) | Km (μM) | kcat/Km (M−1s−1) |
|---|---|---|---|
| NfsA | 43.8±7.0 | 840±260 | 5.2×104 |
| NfsB | 45.3±0.9 | 45±3 | 1×106 |
Initial velocities were monitored at 340 nm at a fixed concentration of NAD(P)H (60 μM NADPH for NfsA, 60 μM NADH for NfsB) and varied concentrations of PT638 (6.25–1500 μM). Data were fit to the Michaelis–Menten equation.
