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. Author manuscript; available in PMC: 2020 Jul 11.
Published in final edited form as: Cell. 2019 Jun 27;178(2):316–329.e18. doi: 10.1016/j.cell.2019.06.003

Figure 5. Bach1 promotes metastasis.

Figure 5.

(A) Quantification of lung metastases at 4 weeks after SQ implantation of KP and KPK cells expressing a GFP/luciferase reporter and transduced with lentiviruses expressing either a dox-inducible shCtrl, shBach1, or shFbxo22 (from left to right, n= 9, 7, 7, 9, 7, and 6). Mice were put on a dox diet 10 days after implantation. Data are presented as means, and squares represent individual data points. (B) Schematic representation of KrasLSL-G12D/+; p53fl/fl (KP) mice intratracheally infected with uSEC lentiviruses containing sgTom, sgKeap1, or sgFbxo22. (C) Metastasis incidence in sgTom, sgKeap1, or sgFbxo22 mice (n= 12, 10, and 9 respectively) at 21 weeks after infection with uSEC lentiviruses. (D) Quantification of lung metastases at 4 weeks after SQ implantation of KP-sgTom and KP-sgBach1 cells expressing a GFP/luciferase reporter and transduced with lentiviruses expressing either a dox-inducible shCtrl or shFbxo22 (from left to right, n= 12, 13, 13, and 12). Mice were put on a dox diet 10 days after implantation. Data are presented as means, and squares represent individual data points. Right, representative brightfield and GFP fluorescent images of lung metastasis. (E) Quantification of lung metastases at 4 weeks after SQ implantation of KP-sgTom and KP-sgFbxo22 cells expressing a GFP/luciferase reporter and transduced with lentiviruses expressing either a dox-inducible shCtrl or shBach1 (from left to right, n= 12, 15, 15, and 14). Mice were put on a dox diet 10 days after implantation. Data are presented as means, and squares represent individual data points. Right, representative brightfield and GFP fluorescent images of lung metastasis.