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. Author manuscript; available in PMC: 2020 Sep 1.
Published in final edited form as: J Steroid Biochem Mol Biol. 2019 Jan 11;192:105283. doi: 10.1016/j.jsbmb.2019.01.001

Figure 6.

Figure 6.

Displacement of [3H]-R1881 Binding from AR in Hela Cells. Cells (4 × 105) were incubated with 0.1 nM [3H]-R1881 for 2 hr in a 96-well plate in the presence and absence of increasing concentrations of drug. Bound [3H]- R1881 from each well was measured by scintillation counting after cell lysis. Non-specific binding was defined as the amount of ligand that was bound in the presence of 10 nM DHT. Displacement by unlabeled R1181 (○); displacement by enzalutamide (◆); displacement by R-biaclutamide (∇); displacement by compound 1 (•); displacement by GTX-560 (∎); displacement by compound 3 (▼); displacement by compound 4 ( ▲ ); and displacement by ASP9521 (●). Mean + SD (n=3)