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Compound 1 (10 – 40 μM) inhibits PSA expression in LNCaP-AKR1C3 cells treated with either 100 nM 4-AD (AKR1C3 substrate) or by 1.0 nM by DHT (AR agonist). PSA expression, AR expression were measured by immunoblot analysis in cell lysates where β-tubulin was used as a loading control.
