Table 2:

Considerations about the target organ/tissue that are needed before printing, in order to determine ideal material and printing properties.

Features	Considerations
Biomimicry	Inculcating relevant biomimicry concepts in the design may aid to enhance its properties and success rate
Cell Density	The density of the target organ/ tissue and the variation seen in it is critical for fabricating the printed part and will determine several parameters such as printing method, viscosity, and rheological properties.
Heterogeneity	The different types of cells that are present and their composition is critical for determining the complexity of fabricating the part, selection of cells and biomaterials, and construct material and design.
Vascularization	Studying how vasculature is present in the target organ/tissue is critical for increasing the viability of the construct
	Construct design should be targeted towards accommodating vasculature, and appropriate layer thickness should be selected.
Innervation	Similar to vasculature, it’s necessary to understand how the nervous system is distributed, especially in tissues where stimulation is critical, such as cardiac tissue.
Engraftment	Using the patient’s stem cells are key to the body accepting the printed part without undesirable local or systemic responses