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. Author manuscript; available in PMC: 2019 Nov 1.
Published in final edited form as: Nature. 2019 May 1;569(7755):265–269. doi: 10.1038/s41586-019-1165-8

Extended Data Fig. 3. Characterization of the sof78 mutation.

Extended Data Fig. 3

a, The seed development (top) and the petal number (bottom) of sof78 mutant and Ler wild type. Photos are representative of at least five independent experiments. b, FLC-LUC bioluminescence signal of indicated plants taken by CCD camera. Data are representative of three independent experiments. c, Expression of spliced FLC relative to UBC in the indicated genotypes. Data are presented as the mean ± SD (n = 4). Asterisk indicates a significant difference (P = 0.0014, two-tailed t test). d, Flowering time of indicated plants grown in long day photoperiod. Data are presented as the mean ± SD (n = 20). Asterisk indicates a significant difference (P < 0.0001, two-tailed t test). e, RT-PCR detection of FLC and UBC transcripts or PCR amplification of indicated fragments from genomic DNA. Data are representative of three independent experiments. f, Flowering time of indicated plants grown in long day photoperiod. Data are presented as the mean ± SD (n = 20). Asterisk indicates a significant difference (P < 0.0001, two-tailed t test). g, Flowering time of indicated plants grown in long day photoperiod. Data are presented as the mean ± SD (n = 8). Asterisks indicate significant differences (P ≤ 0.0077, two-tailed t test). h, Genomic FLL2 locus indicating upstream and downstream genes and positions of sof78 mutation and fll2-2 T-DNA insertion (top); illustration of transgenic FLL2-eYFP construct (bottom). Thick black boxes indicate exons, thin black boxes indicate UTRs and black lines indicate introns. i, RT-PCR detection of FLL2 and UBC transcripts in Col-0 and fll2-2. Data are representative of three independent experiments. j, Expression of spliced FLC relative to UBC in the indicated genotypes. Data are presented as the mean ± SD (n = 4). k, Flowering time of indicated plants grown in long day photoperiod. Data are presented as the mean ± SD (n = 12). l, Phylogenetic tree of FLX family proteins. The tree was drawn by PHYLIP program. Bootstrap values from 1000 trials are shown. m, FLC-LUC bioluminescence signal of indicated plants taken by CCD camera. Data are representative of three independent experiments. n, Flowering time of indicated plants grown in long day photoperiod. Data are presented as the mean ± SD (n = 10). Asterisk indicates a significant difference (P < 0.0001, two-tailed t test).