Extended Data Fig. 5. Characterization of FLL1 and FLL3.
a, Colocalization of FLL1-YFP and FLL3-YFP with FCA-CFP in tobacco leaf nuclei. Scale bars, 5 μm. Data are representative of three independent experiments. b, c, Top, protein domain structures of FLL1 and FLL3. Bottom, predictions of prion-like domains and disordered regions by PLAAC and D2P2 algorithms, respectively. d, Interactions of FCA with FLLs in yeast two-hybrid assay. Combinations of constructs were transformed into yeast AH109 strain and assayed on stringent medium. Three independent colonies were tested. E.V., empty vector. Data are representative of three independent experiments.