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. 2019 Jul 12;9:10127. doi: 10.1038/s41598-019-46470-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Aggregation of mHTT in the abdominal FB in vivo. Abdominal FB dissected from 25QHTT^ex1 and 120QHTT^ex1 females at different ages were probed with anti-HTT antibody to reveal the accumulation and/or aggregation of HTT. (a) Cg-GAL4 driven-mHTT exon1 peptides form aggregates in the abdominal FB. At day3, aggregates begin to appear as bright puncta (green) in 120QHTT^ex1 flies (right panel). Along with the aggregates, soluble diffused forms are also detected in the FB at this time point. On the other hand, FB of 25QHTT^ex1 does not show any accumulation of unexpanded HTT exon1 throughout the indicated ages (left panel). (n = 6/genotype/age) Scale bar, 10 µm. (b) The image analysis of the FB from 120QHTT^ex1 flies (a, right panel) is shown as intensity profiles of HTT (black) and DAPI (gray) at different ages. The intensity of HTT signal is lowest at day0 and higher at day10 while the intensity of DAPI signal is relatively constant throughout. (c) The number of detectable aggregates per cell was determined by analysing the 3D stacks of all the images in (a) using the ImageJ software (n = 6/genotype/age). The objects i.e. apparent aggregates are detected strongly in the FB from 120QHTT^ex1 flies (a, right panel) while the FB from 25QHTT^ex1 generates negligible signal (a, left panel). The number of objects detected in the day0 old FB from 120QHTT^ex1 flies specimens is notably less than the number of objects in the following days.