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. 2019 Jul 12;10:3085. doi: 10.1038/s41467-019-11083-2

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Fig. 4 — Hill cooperativity analysis of the ATPase activity of human DDX3X. a ATPase activity of DDX3X was stimulated by 100 nM 22-bp double-stranded RNA (dsRNA) with a 25-nt 3ʹ overhang (Strand 1: 5ʹ-UCGUGGCAUUUCUGCGUCGUUCUUUUCUUUUCUUUUCUUUUCUUUUC-3ʹ; Strand 2: 5ʹ-GAACGACGCAGAAAUGCCACGA-3ʹ) at indicated concentrations of the helicase. Solid line is the best fits to the Hill equation (V_max = 7.4 ± 0.3 μM min⁻¹, K_1/2 = 320 ± 20 nM, H = 1.9 ± 0.2). b ATPase activity of DDX3X was stimulated by 100 nM 16-bp dsRNA with a 25-nt 3ʹ overhang (Strand 1: 5ʹ-GCGUCUUUACGGUGCUUAAAACAAAACAAAACAAAACAAAA-3ʹ; Strand 2: 5ʹ-AGCACCGUAAAGACGC-3ʹ) at indicated concentrations of the helicase. Solid line is the best fits to the Hill equation (V_max = 3.1 ± 0.4 μM min⁻¹, K_1/2 = 895 ± 120 nM, H = 1.7 ± 0.3). Error bars represent 1 SD. Source data are provided as a Source Data file