Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Jul 12;9:10131. doi: 10.1038/s41598-019-46171-2

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Characterization of MK components in human cells and Xenopus oocytes. Image overlays (fluorescence and DIC) of HEK293 cells transfected with LII reporter plasmids expressing mCherry (A) and NLS-YFP (B) driven by the ELF-1α promoter. (C) Construct design for functional expression of the KAT1 K⁺ channel in Xenopus laevis oocytes. Three independent DNA sequences placed downstream of the β-Globin 3′UTR were tested in parallel to monitor their effect on channel expression. Unique enzyme sites for plasmid linearization prior to cRNA synthesis are shown. (D) Upper panel: Confocal fluorescence images of oocytes injected with KAT1-YFP cRNA followed by the 3′ sequences shown in (B) (scale bars: 200 μm). Lower panel: Current voltage curves and representative current traces of oocytes micro-injected with KAT-YFP cRNA as indicated. (E) Steady state currents for the corresponding curves in (D). Shown are the measurement means and standard deviations. Voltage steps ranged from +60 mV to −160 mV in 20 mV decrements. Holding potential was set to −40 mV. Scale bars in (A) represent 10 µm and 200 µm in (D).