. Author manuscript; available in PMC: 2019 Jul 13.

Published in final edited form as: Niger Postgrad Med J. 2019 Apr-Jun;26(2):80–86. doi: 10.4103/npmj.npmj_59_19

Table 1:

Sequences of primers for hepatitis B virus amplification and genotyping used by multiplex-nested polymerase chain reaction

Primer	Sequence (5’–3’)	Specificity	Position	Polarity
1^st round PCR
PI	TCACCATATTCTTGGGAACAAGA	Universal	2823–2845	Sense
Sl-2	CGAACCACTGAACAAATGGC	Universal	685–704	Antisense
2^nd round PCR: Mix A
B2	GGCTCCAGTTCCGGAACAGT	Type A-E	67–86	Sense
BA1R	CTCGCGGAGATTGACGAGATGT	Type A	113–134	Antisense
BB1R	GGTCCTAGGAATCCTGATGTTG	TypeB	165–186	Antisense
BC1R	CAGGTTGGTGAGCTGGAGA	TypeC	2979–2996	Antisense
2^nd round PCR: Mix B
B2R	GGAGGCGGATTTGCTGGCAA	Type D-F	3078–3097	Antisense
BD1	GCCAACAAGGTAGGAGCT	Type D	2979–2996	Sense
BE1	CACCAGAAATCCAGATTGGGACCA	Type E	2955–2978	Sense
BF1	GTTACGGTCCAGGGTTACCA	Type F	3032–3051	Sense