Table 1.

Et-EmAMA1 selection based on EYFP expression

Generations	% EYFP expression	Selection strategy
1	32.3	Drug
2	78.3	Drug + FACSa
3	93.2	Drug + FACS
4	96.5	Drugb
5	94.7	Drug
6	94.2	–c

^aFacilitating recombinant Eimeria parasite selection by pyrimethamine combined with fluorescence-activated cell sorting (FACS)

^bStabilization of the recombinant Eimeria population by pyrimethamine

^cThe recombinant Eimeria population was stable without selection pressure