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. Author manuscript; available in PMC: 2019 Jul 13.
Published in final edited form as: J Tissue Eng Regen Med. 2014 Nov 25;11(3):658–668. doi: 10.1002/term.1962

Figure 1.

Figure 1.

Schematic representation of bioengineered recombinant tooth bud construct fabrication. Postnatal dental recombinant constructs were fabricated from M3 and M4 molar porcine dental epithelium (DE) and differentiation medium (DM) tissues (pDE-T, pDM-T), human dental pulp tissues and cells (hDM-T and hDM-C), and human gingival epithelial cell suspensions (hGE-C) as indicated. Tissue-tissue (T + T) recombinant constructs were sutured together, while cultured cells were resuspended in collagen and pipetted over tooth tissues to generate C + T constructs, or layered together to generate the cell-cell (C + C) constructs. Dental tissues layered with acellular collagen scaffold (S) were used as tissue alone controls.