Skip to main content
. 2019 Jul-Sep;11(3):259–263.

Figure 2.

Figure 2.

In vitro DNA cleavage using Cas9 nuclease and crRNAs (CRISPR RNP). A) Cas9 nuclease and crRNAs for targeting RAG1, RAG2 and IL2RG genes. Left: incubation at 37°C after 1 hr. Lane1, 2: first bands are the Rag2 uncut PCR products, second and third bands are cleaved fragments represented by white arrows. Lane 3, 4: first bands are the IL2RG uncut PCR products, second and third bands are cleaved fragments represented by white arrows. Lane 5: first band is the Rag1 uncut PCR product, second band is one of the cleaved fragments, another cleaved fragment of Rag1 disappeared on agarose gel electrophoresis but after 2 hr, a faint band was found. Lane 6: Rag1 PCR product as a DNA control. Right: incubation at 37°C after 2 hr. The lanes are in the same order just after 2 hr. B) Cas9 nuclease and crRNAs for targeting RAG2 genes after 6 months of shelf life. Very faint bands show inefficient targeting by Rag2 crRNAs. In all agarose gel pictures, bands resolution is not brilliant due to low quality of GelRed.