Table 1.
The effect of supplementation of semen extender with antioxidants on sperm motility parameters in frozen–thawed stallion sperm
| Parameters | Control | Sericin | Glutathione | Sericin+Glutathione |
|---|---|---|---|---|
| Motile spermatozoa (%) | 44.74±5.49 | 49.89±4.06 | 44.10±5.50 | 49.30±6.70 |
| Progressive spermatozoa (%) | 32.53±7.20 | 38.57±5.50 | 30.92±7.00 | 37.48±9.10 |
| Class A (%) | 19.90±5.70 | 20.28±6.20 | 18.43±7.02 | 23.32±8.50 |
| Class B (%) | 12.64±1.65 ab | 18.29±2.30 a | 12.50±0.59 b | 13.76±1.99 ab |
| Class C (%) | 12.20±2.00 | 11.31±2.27 | 13.18±2.00 | 11.83±2.50 |
| Class D (%) | 55.27±5.40 | 50.11±4.06 | 55.90±5.59 | 50.70±6.70 |
| VCL (µm/s) | 34.97±5.57 | 36.34±5.15 | 34.87±5.70 | 37.91±7.49 |
| VSL (µm/s) | 17.48±3.98 | 19.76±4.42 | 16.73±5.21 | 22.19±7.01 |
| VAP (µm/s) | 21.77±4.22 | 24.07±4.54 | 20.98±5.37 | 26.5±7.16 |
| MAD (°) | 10.56±3.99 | 8.94±2.51 | 8.74±2.35 | 8.33±2.55 |
| ALH (µm) | 1.94±0.16 | 2.05±0.12 | 1.95±.19 | 1.97±0.20 |
| BCF (Hz) | 0.31±0.12 | 0.29±0.10 | 0.27±0.11 | 0.32±0.13 |
| LIN (%) | 32.91±4.38 | 39.73±4.05 | 31.44±6.14 | 38.08±7.80 |
| WOB (%) | 45.78±4.15 | 52.97±3.57 | 44.69±5.98 | 50.56±7.16 |
| STR (%) | 48.90±5.19 | 55.10±3.86 | 47.96±6.32 | 51.92±7.62 |
VCL: Curvilinear velocity, VSL: Straight line velocity, VAP: Average path velocity, MAD: Mean angular displacement, ALH: Amplitude of lateral head displacement, BCF: Beat cross frequency, LIN: Linearity index (LIN= VSL/VCL×100), WOB: Wobble (WOB=VAP/VCL×100), and STR: Straightness index (STR=VSL/VAP×100).
a, b)
Different superscripts within the same row demonstrate significant difference (p<0.05).