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. Author manuscript; available in PMC: 2019 Dec 5.
Published in final edited form as: Nature. 2019 Jun 5;570(7762):528–532. doi: 10.1038/s41586-019-1276-2

Extended Data Figure 8. Sorting purity of mouse ILCs.

Extended Data Figure 8.

(a) B6 mice were aerosol infected with ~100 CFU Mtb and lungs were harvested at 5 dpi. Lung CD45 population was enriched by using CD45 Microbeads. CD45 enriched cells were stained and lung ILCs (CD45+CD127+Lin−NK1.1−) were purified by using FACSJazz. Sort purity is shown here. (b) mRNA expression of Ccr6, Rorγt and Ahr relative to GAPDH on the purified ILCs (CD45+CD127+Lin−NK1.1−) and non ILC population (CD45+CD127−Lin−NK1.1−) were quantitated by RT-PCR. Significance by two way ANOVA, ****p<0.0001.