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. Author manuscript; available in PMC: 2020 Aug 1.
Published in final edited form as: J Periodontal Res. 2019 Feb 25;54(4):444–452. doi: 10.1111/jre.12646

Figure 4. Effect of vitamin D on IL-1α mRNA levels in vitro (A) and in vivo (B).

Figure 4.

A. Cultured OKF6/TERT1 cells were treated with either 10nM 1,25(OH)2D3 or 0.1% ethanol (vehicle control) for 24 hours. Total mRNA was isolated and levels of IL-1α mRNA were quantified by QRT-PCR. B. Wild-type C57Bl/6 mice (n=5 per group) were treated with 50μl mineral oil containing either 1μM vitamin D3, 1,25(OH)2D3 or vehicle control. After 6 hours, gingivae were excised, total mRNA was isolated and IL-1α mRNA levels were quantified by QRT-PCR relative to β-actin. Data are shown as mean expression relative to control +/− SEM. Differences are significant, *p<0.0001, by t-test.