Figure 4.
Pharmacological properties of the nicotine-evoked current in human DRG neurons. Slow currents were evoked with nicotine for 500 ms (indicated by the bar above each trace) before and after the application of mecamylamine (MEC, 30 μM), a nAChRs antagonist (A), methyllycaconitine citrate (MLA, 20nM), an α−7 subunit selective antagonist (B), HC-030031(HC-03, 30μM), a TRPA1 receptor antagonist (C). (D) Neurons were also challenged with cytisine (100 μM) for 500 ms (indicated by the bar above the figure). The concentration of nicotine applied was indicated at left side of the traces.