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Visualization of NP8-induced degradation. (A) The EGFP-HDAC6 (green) was localized in the cytoplasm of HeLa cells. Scale bar: 10 µm. (B) NP8 treatment caused reduced fluorescence signal within 48 h. Nuclei were labeled by Hoechst 33342 (blue). Scale bar: 50 µm. (C) Confirmation of EGFP-HDAC6 degradation in (B) by immunoblotting. (D) Relative mean fluorescence intensity of representative single cells was monitored via confocal microscope from 24 h to 36 h post 5 µmol/L NP8 treatment
