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. 2018 Jun 28;10(8):566–582. doi: 10.1007/s13238-018-0559-y

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Uninjured SCID mice displayed boosted synaptic transmission program. (A) Immunostaining of SNAP25 and SMI-312 in WT/SCID uninjured spinal cord samples with high magnification images of indicated areas presented to the right. SNAP25⁺ puncta within SMI-312⁺ labeled axons (per µm³) of uninjured samples in the equivalent location as injured samples were measured (mean ± SEM; n ≥ 20, ***P < 0.001, Mann Whitney test). (B) Western blotting analysis of blue module hub genes VAMP1, SNAP25, RAB3A, STMN2 and β-actin protein levels of uninjured spinal cord sample from WT and SCID mice (mean ± SD; n = 4 (WT), n = 5 (SCID), *P < 0.05, **P < 0.01, unpaired Student’s t-test)