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Green fluorescence (SYTO 9) and red fluorescence (PI) plot of the bacterial cells from liquefied stool samples prepared by (A) a standard stool-liquefaction process and (B) our acoustofluidic stool liquefier. SYTO 9, a membrane permeable stain, is employed to identify live bacterial cells while PI, a membrane impermeable stain, is used to identify dead bacterial cells. The percentage of live bacterial cells was 39.1% and 45.1% for (A) and (B), respectively.
