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. Author manuscript; available in PMC: 2019 Jul 14.
Published in final edited form as: Nat Chem Biol. 2018 Dec 10;15(1):62–70. doi: 10.1038/s41589-018-0177-2

Figure 4: Monitoring of the Q93 residue during UBE2A catalysis.

Figure 4:

(1H,15N)-HSQC spectra of (A) 15N-C88S-UBE2A during oxyester linkage formation and (B) 15N-WT- UBE2A during thioester linkage formation, showing the peaks correspondent to Q93 backbone and Q93 sidechain. Black - before ATP addition, red - right after ATP addition, cyan - 5 hours after ATP addition.