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Comparison between WT-, Q93E- and Q93R-UBE2A enzymes in the ability of monoubiquitinate PCNA. (A) Anti-PCNA western blot (uncropped blots are shown in Supplementary Figure 11). PCNA-Ub - monoubiquitinated PCNA. (B) Graph showing the formation of ubiquitinated PCNA by each enzyme, quantified by ImageJ. Three independent experiments were conducted and mean ± standard deviations are indicated.
