Figure 2.

Comparison of the 2D spectra (HN left; CON right) acquired through the CON//HN experiment on ¹³C, ¹⁵N-labeled α-synuclein at 310 K in different experimental conditions: (A) purified sample in 100 mM NaCl, 50 μM EDTA, and 20 mM phosphate buffer at pH 7.4 is shown; (B) in Escherichia coli cells lysate resuspended in the same buffer as in (A) and (C) in-cell. The traces of a representative signal extracted from the HN (red; left) and the CON (blue; right) spectra are also reported. To see this figure in color, go online.