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Domain swapping of RU variants assayed by SDS-PAGE. (A) 4–15% SDS-PAGE gel illustrating domain-swapped and monomeric (39.3 kDa) RU proteins is shown. (B) The monomeric region of 4–20% polyacrylamide gel is shown, indicating that the protein monomers, like the domain-swapped complexes, remain folded in SDS.
