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. 2019 Jun 7;117(1):56–65. doi: 10.1016/j.bpj.2019.06.002

Table 1.

Binding Affinities and Association Rates of Homo Complexes, Overlap Complexes, and Gap Complexes

RBP1–59 RBP1–69 RBP1–84 RBP1–96 RBP1–124
RBP60–277 104 ± 4 1.62 ± 0.05 2.4 ± 0.3 <0.1 4.0 ± 0.1
119 ± 16 780 ± 40 1480 ± 60 (2.90 ± 0.05) × 105 530 ± 20
RBP70–277 ND 4.0 ± 0.1 7.2 ± 0.8 <0.1 2.7 ± 0.1
340 ± 30 900 ± 60 (2.54 ± 0.04) × 105 620 ± 20
RBP85–277 ND 5.3 ± 0.2 9.3 ± 0.4 <0.1 4.5 ± 0.5
540 ± 30 1490 ± 70 (2.91 ± 0.04) × 105 620 ± 30
RBP97–277 ND ND 79 ± 30 <0.1 3.0 ± 0.5
590 ± 40 (2.13 ± 0.04) × 105 570 ± 10
RBP125–277 ND ND ND 2.98 ± 0.47 0.45 ± 0.05
(4.26 ± 0.08) × 105 1260 ± 30

Kd (units of nM) and kon (italics; units of M−1 s−1) are listed at the top and bottom of each cell, respectively. Kd errors are SDs of three independent measurements. kon errors are those calculated from the linear regression fits (three independent data sets). ND, binding not detected. indicates homo complexes (diagonal). Overlap complexes are above the diagonal. Gap complexes are below the diagonal.