Figure 4.

Comparison of the partitioning free energies of the terminal strand mutants with reported hydrophobicity scales. Partitioning free energies derived for only the terminal strand substituents have been plotted as a function of depth of lipid-facing host sites at which cysteine has been incorporated. The estimated energetic cost of substitution (Xxx→Cys) calculated from a previously reported depth-dependent computational transfer free energy scale (Liang scale) (38) (left panel) and an empirical potential for amino acid insertion into the membrane (DeGrado scale) (7) (right panel) are depicted as gray histograms. To see this figure in color, go online.