Table 6.

Genetic targets for the PCR-based identification of cestode parasites.

Symbol (bold) and aliases	Primer designations	Primer sequence (5‘-3‘) and approximate amplicon size	Reference and primer code [in squared brackets] as referred to in Table 2, Table 3, Table 4, Table 5
Target gene: Mitochondrial cytochrome c oxidase subunit 1
mt-CO1 cox1, COX1, COI	JB3 JB4.5	5'-TTT TTT GGG CAT CCT GAG GTT TAT-3' 5'-TAA AGA AAG AAC ATA ATG AAA ATG-3' 529 bp	Bowles et al. (1992). [P1]
	F/COI R/COI	5'-TTG AAT TTG CCA CGT TTG AAT GC-3' 5'-GAA CCT AAC GAC ATA ACA TAA TGA-3' 880 bp	Nakao et al. (2000). [P2]
Target gene: Mitochondrial NADH:ubiquinone oxidoreductase core subunit 1
mt-ND1 nd1, ND1, NAD1	JB11 JB12	5'-AGA TTC GTA AGG GGC CTA ATA-3' 5'-ACC ACT AAC TAA TTC ACT TTC-3' 529 bp	Bowles and McManus (1993). [P3]
	NAD1-FF NAD1-RR	5'-ATT GGK TTA TTT CAG AGT TTT TCT GAT TTA-3' 5'-CTC MCC ATA ATC AAA TGG ACT ACG-3' 394 bp	Eberwein et al. (2013). [P4]
Target gene: Mitochondrial 12S rRNA
mt-RNR1 12S	60.for 375.rev	5'-TTA AGA TAT ATG TGG TAC AGG ATT AGA TAC CC-3' 5'-AAC CGA GGG TGA CGG GCG GTG TGT ACC-3' 357 bp	von Nickisch-Rosenegk et al. (1999). [P5]
	Cest3 Cest5	5'-YGA YTC TTT TTA GGG GAA GGT GTG-3' 5'-GCG GTG TGT ACM TGA GCT AAA C-3' 267 bp	Trachsel et al. (2007). [P6]
	CES12SF CES12SR	5'-AGG GGA TAG GAC ACA GTG CCA GC-3' 5'-CGG TGT GTA CMT GAG YTA AAC-3' 480 bp	Goldberg et al. (2014). [P7]
	12S Taenia FF 12S Taenia RR	5'-CAC AGT GCC AGC ATC YGC GGT-3' 5'-GAG GGT GAC GGG CGG TGT GTA C-3' 426 bp	Eberwein et al. (2013). [P8]
Target gene: Ribosomal Internal Transcribed Spacer
ITS2its2	NC-2 NC-6	5'-TTA GTT TCT TTT CCT CCG CT-3' 5'-ATC GAC ATC TTG AAC GCA CAT TGC-3' 600-700 bp	Gasser and Chilton (1995). [P9]