Figure 5.

Gene function annotation. The sequence reads were assigned to the SEED subsystems by Megan 6. (A) Clustering of SEED subsystems in all GGN and normal tissue samples. The sequence reads were normalized by z-scores of GGN samples. Four samples clusters and 4 subsystem clusters were shown by the z-score profiles. However, the sample clusters show neither classifications of GGN types nor different patients. (B) Comparing the gene function annotation between GGNs and normal samples by t-test. Significances were found between the Secondary Metabolism of SEED annotation in GGNs (SM GGN) and in normal sample (SM NOR) and between the COG0515 Serine Threonine protein kinase annotated of eggNOG in GGN (STK GGN) and in normal control tissues (STK NOR). GGN, ground-glass nodule.