Figure 3.

The Use of Median Values and Interquartile Ranges Is More Appropriate for Presenting Highly Variable Bioluminescence Data

(A and B) The mean (± SEM) (A) and median (± interquartile range) (B) bioluminescence signals obtained after intradermal electroporation of luciferase encoding sr-mRNA in the absence or presence of RNase inhibitor. Five micrograms of luciferase encoding sr-mRNA dissolved in 50 μL PBS was injected in the flanks of mice and immediately electroporated. The green curves show the luciferase expression of the sr-mRNA without supplementation with RNase inhibitor. The blue and red curves depict the luciferase expression after adding, respectively, 0.33 and 1.0 U/μL RNase inhibitor to the sr-mRNA just before injection. The black curves represent the luciferase expression after adding 1.0 U/μL RNase inhibitor before storage at −80°C. (n = 6). Due to the high variation in luciferase expression, no significant differences were observed between the groups.