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. 2019 Jun 13;13(1):10–20. doi: 10.1016/j.stemcr.2019.05.013

Figure 1.

Figure 1

s-SHIP/GFP Expression Is Detected in Mammary Tumors of s-SHIP-GFP/C3(1)-Tag Bitransgenic Mice

(A) H&E staining of paraffin-embedded sections of mammary tumors illustrating different stages of tumor development: a, ductal hyperplasia; b, ductal carcinoma in situ; c, infiltrative solid carcinoma; d, solid carcinoma. Scale bars, 50 μm (a, b, d) and100 μm (c). See also Figure S1A.

(B) Representative images (n = 4) of frozen sections of mammary tumors containing GFP+ cells (green) and stained with cytokeratin 14 (K14, red, upper panels), cytokeratin 8 (K8, red, lower panels), and DAPI nuclear stain (blue) Scale bars, 100 μm (left panels) and 10 μm (right panels). Images of 7-week-old mammary gland stained with K14 and K8 are shown in Figure S1B.

(C) Representative flow cytometry analysis (n = 5) of GFP expression in dissociated mammary cancer cells isolated from a 5-month-old bitransgenic mouse. P1 gate is set as to exclude dead cells (Zombie violet+). See also Figure S1C.

(D) Representative flow cytometry analysis (n = 3) of total Lin− cells, Lin−GFP−, and Lin−GFP+ cells from two different tumors: T1 and T2. Cells were analyzed for the expression of CD24, CD29, CD49f, and EpCAM cell-surface markers. See also Figure S1D.