CCL5 and CCR5 Expression Are Increased following Ionizing Irradiation
(A) ELISA of CCL5 expression from C57BL/6 ECs at 0 (Non-irrad), 2, and 24 h following 800 cGy irradiation. n = 6–8 per group, ∗p = 0.03 and p < 0.0001 for 2 and 24 h compared with nonirradiated ECs, respectively.
(B) Ccr5 mRNA expression of C57BL/6 KSL cells at 2 h following 300 cGy compared with nonirradiated KSL cells. Data are normalized to nonirradiated control samples and GAPDH. n = 8 per group, ∗p = 0.002.
(C) CCR5 protein expression by flow cytometric analysis of whole bone marrow (WBM), Lin−, and KSL cells following 300 cGy over time compared with nonirradiated control mice (0 h). n = 3–5 per group per time point, ∗p < 0.0001 for times 0, 2, 4, and 24 h.
(D) Representative flow cytometric analysis and quantification of CCR5 expression in BM Lin− cells at 24 h following 300 cGy compared with nonirradiated control mice. n = 5 per group per time point, ∗p < 0.0001. SSC, side scatter. Data are shown as means ± SEM. Student's t test (two-tailed with unequal variance) was applied to the datasets except in (C) where a one-way ANOVA analysis was applied.